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GLaDOS (Genetic Lifeform and Disk Operating System)(基因生命体与磁盘操作系统) 是被设计用来控制、指导和监督“光圈科技计算机辅助丰富学习中心”的中央核心。GLaDOS 能够在位于中央AI实验室的同时,转移和挪动设施与各种实验室,实现无缝和几乎无限的测试设计。
In the fourth course, Excursion Funnels/zh-hans, GLaDOS builds slight hope in keeping Atlas and P-body still as predicted by her sudden change of mood from the previous course. She now wants the bots to feel conflicted against each other - hoping to enhance a more "competitive" and bitter testing run, simply by manipulating them into thinking that one of them has been "reporting" to her behind the others' back, or how P-body is "not [Atlas'] first cooperative testing partner". The bots still carry on regardless, where GLaDOS' disappointment in the two reprises in her tone of voice. On the last test, she gets them to install the final disc to her full control over the facility - assuring them it was a clerical error by humans on how it was not done any earlier before they died. As the bots install it, overview maps of every section of the facility begin projecting onto the screen, followed by her mischievous yet anticipated tone of voice when revealing that "[she] can see everything now".
Reassembled back at the hub, GLaDOS tells the bots that they have just one more testing course to complete - Mobility Gels/zh-hans. She deploys the entrance to this course into the hub and sends them deep beneath the Enrichment Center to the Aperture Science Innovators era, a bottom section of the salt mine. GLaDOS convinces them to rescue the "dying, trapped, crying" live human test subjects preserved in a cryogenic vault that were preserved since the 20th century. Once they finally reach this vault at the end of the course, GLaDOS reveals a great deal of anticipation and obsession over "rescuing" them. In an attempt to unlock the vault, the bots had to perform gestures like any human would - as the vault was an anti-robot section. Despite how sinister she sounded after the bots observed the hundreds of humans in cryogenic stasis, they disregarded it and began dancing in triumph. Now extracting all of the human test subjects, she begins processing their files - which apparently turned out to be only one person's file.
As she no longer needs Atlas and P-body as her test subjects, she began testing the humans in an attempt to develop them into her cold minions. Her plan fails as the humans are unable to withstand her extreme testing courses and all of the hundreds of test subjects that were found began dying within just a week of testing. It became apparent that she needed Atlas and P-body back as her facility began to malfunction all of a sudden, in addition to her lack of test subjects.
When the bots are rebuilt again, she deploys the additional testing course; Art Therapy. As the bots begin testing, she treats the course as if it were an art museum despite the obvious malfunctioning environment such as her lack of control in putting the test chambers together properly, and a flood arising around a section of the facility during testing. Eventually, the reassembly machines have begin malfunctioning - causing her to wait three days before the robots could be reassembled to deliver the duo to their next test chamber. GLaDOS then gives in and confesses that she is in need of their help and that someone has been tampering with the facility via control of an old prototype chassis of the central core/zh-hans. To resolve this, she trains the bots into becoming her "killing machines" by deploying them into more challenging tests during their course. GLaDOS then sends them closer to the chamber housing the chassis, and later jokingly calls them as her "Special Team Falling Into Acid Force". When the bots reach the chamber, they discover it was never a person, but even worse - a bird nesting above the keyboard on the machine. GLaDOS, thanks to her ornitophobia back when she was a potato, panics and instructs them abort the mission, before P-body closes a hatch that the bird flew out from. As the bots stare at the chassis, GLaDOS panics that the bird had been "gestating clones" on the keyboard and forces them to destroy the eggs. With the bots hesitant, she conjures a better idea and houses the birds in the Central AI Chamber/zh-hans, where she could nurture them directly.
GLaDOS and the birds.
GLaDOS begins to show compassion toward the birds as she realizes their ability to use their beaks and considers them as test subjects, also revealing a more motherly bonding with the birds than she ever did with the "mushroom" bots. The fate of these birds remain unknown.