GLaDOS

GLaDOS, known in full as the Genetic Lifeform and Disk Operating System, is the computer system designed to control and oversee Aperture Laboratories, as well as the guide of the Aperture Science computer-aided Enrichment Center. She has the ability to shift and move the facilities and the chambers, making for seamless and almost infinite testing design.

Trivia

 * GLaDOS is voiced by Ellen McLain, who also voices the Administrator in Team Fortress 2 and the Combine Overwatch in the Half-Life 2 series.